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Vector Laboratories abc kit
Abc Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 12752 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vectastain+abc+elite+standard+kit/VECTASTAIN+Elite+ABC+HRP+Kit+(Peroxidase%2C+Standard)/pmc12887268-371-2-4
Average 99 stars, based on 12752 article reviews

abc kit - by Bioz Stars, 2026-09

99/100 stars

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Immunohistochemistry:

Article Title: Acid Ceramidase Deficiency in Mice Leads to Severe Ocular Pathology and Visual Impairment
Article Snippet: .. For immunohistochemistry (IHC), the following primary antibodies, secondary antibodies, and staining reagents were used: rat anti-mouse Mac-2 at 1:8000 (galectin-3 clone M3/38; Cedarlane, Burlington, ON, Canada); goat anti-mouse cathepsin D at 1:3000 (Santa Cruz Biotechnology, Dallas, TX); biotinylated rabbit anti-rat IgG antibody at 1:5000 (Vector Laboratories, Burlingame, CA); biotinylated donkey anti-rabbit IgG antibody at 1:500 (Vector Laboratories); avidin-biotin/horseradish peroxidase (Vector Laboratories); 3,3′-diaminobenzidine kit (Vector Laboratories); and Vectastain ABC Elite Standard kit (Vector Laboratories). .. For immunofluorescence staining, the following primary antibodies were used: rabbit anti-ionized, calcium-binding adapter molecule 1 (Iba1) at 1:2000 (Wako Chemicals USA, Cambridge, MA) and chicken anti–glial fibrillary acidic protein (GFAP) at 1:2000 (Aves Lab Inc., Tigard, OR).

Article Title: Acid Ceramidase Deficiency in Mice Leads to Severe Ocular Pathology and Visual Impairment.
Article Snippet: .. For immunohistochemistry (IHC), the following primary antibodies, secondary antibodies, and staining reagents were used: rat anti-mouse Mac2 at 1:8000 (galectin-3 clone M3/38; Cedarlane, Burlington, ON, Canada); goat anti-mouse cathepsin D at 1:3000 (Santa Cruz Biotechnology, Dallas, TX); biotinylated rabbit anti-rat IgG antibody at 1:5000 (Vector Laboratories, Burlingame, CA); biotinylated donkey anti-rabbit IgG antibody at 1:500 (Vector Laboratories); avidin-biotin/horseradish peroxidase (Vector Laboratories); 3,30-diaminobenzidine kit (Vector Laboratories); and Vectastain ABC Elite Standard kit (Vector Laboratories). .. For immunofluorescence staining, the following primary antibodies were used: rabbit anti-ionized, calcium-binding adapter molecule 1 (Iba1) at 1:2000 (Wako Chemicals USA, Cambridge, MA) and chicken antieglial fibrillary acidic protein (GFAP) at 1:2000 (Aves Lab Inc., Tigard, OR).

Article Title: Chronic lung injury and impaired pulmonary function in a mouse model of acid ceramidase deficiency.
Article Snippet: .. For immunohistochemistry, the following primary and secondary antibodies and reagents were used: rat antimouse neutrophil (clone 7/4; Cedarlane, Burlington, ON, Canada), rat anti-mouse Mac-2 (Galectin-3 clone M3/38; Cedarlane), rabbit antiPro-SP-C (Sevenhills Bioreagents, Cincinnati, OH), biotinylated rabbit anti-rat (Vector, Burlingame, CA), biotinylated goat anti-rabbit IgG (Vector), avidin-biotin/horseradish peroxidase (HRP) (Vector), diaminobenzidine kit (Vector), and Vectastain ABC Elite Standard kit (Vector). ..

Staining:

Article Title: Cell-specific wiring routes information flow through hippocampal CA3
Article Snippet: .. For DAB staining (Vectastain ABC-Elite Standard kit, Vector Laboratories PK-6100), slices were fixed in a solution containing 2.5% paraformaldehyde (PFA, TAAB Laboratories Equipment), 1.25% glutaraldehyde (GA; CarlRoth, 4157.1), and 15% (v/v) saturated picric acid solution (Sigma-Aldrich, P6744-1GA). .. After washing with phosphate buffer (PB; 0.1 M NaH 2 PO 4 and 0.1 M Na 2 HPO 4 , Merck; titrated to pH 7.35), samples were treated with hydrogen peroxide (1%, 10 min; Sigma-Aldrich, 95321-100ml), permeabilized with 2% Triton X-100 (in PB, Sigma-Aldrich) for 1 h, and transferred to a solution containing 1% avidin-conjugated horseradish peroxidase complex and 1% Triton X-100 for ∼12 h. After rinsing in 0.1 M PB, slices were incubated with a solution containing 0.036% DAB (Sigma-Aldrich, D5637-5G), 0.006% NiCl 2 (Sigma-Aldrich, 223387-25G) and 0.008% CoCl 2 (Sigma-Aldrich, C8661-25G), and developed by addition of 0.01% hydrogen peroxide.

Article Title: Cell-specific wiring routes information flow through hippocampal CA3.
Article Snippet: .. For DAB staining (Vectastain ABC-Elite Standard kit, Vector Laboratories PK-6100), slices were fixed in a solution containing 2.5% paraformaldehyde (PFA, TAAB Laboratories Equipment), 1.25% glutaraldehyde (GA; Carl Roth, 4157.1), and 15% (v/v) saturated picric acid solution (Sigma-Aldrich, P6744-1GA). .. After washing with phosphate buffer (PB; 0.1 M NaH2PO4 and 0.1 M Na2HPO4, Merck; titrated to pH 7.35), samples were treated with hydrogen peroxide (1%, 10 min; Sigma-Aldrich, 95321-100mL), permeabilized with 2% Triton X-100 (in PB, Sigma-Aldrich) for 1 h, and transferred to a solution containing 1% avidin-conjugated horseradish peroxidase complex and 1% Triton X-100 for ∼12 h. After rinsing in 0.1 M PB, slices were incubated with a solution containing 0.036% DAB (Sigma-Aldrich, D5637-5G), 0.006% NiCl2 (Sigma-Aldrich, 223387-25G) and 0.008% CoCl2 (SigmaAldrich, C8661-25G), and developed by addition of 0.01% hydrogen peroxide.

Avidin-Biotin Assay:

Article Title: Human hippocampal CA3 uses specific functional connectivity rules for efficient associative memory.
Article Snippet: A subset of rodent samples were imaged using DAB (3,30-diaminobenzidine-tetrahydrochloride hydrate) staining, where fixation solution contained 2.5% PFA, 1.25% glutaraldehyde (GA; Carl Roth, 4157.1), and 15% (v/v) saturated picric acid solution (SigmaAldrich, P6744-1GA) in PB. .. After washing with PB, samples were treated with hydrogen peroxide (1%, 10 min; Sigma-Aldrich, 95321–100ml), permeabilized with 1% Triton X-100 (in PB) for 1 h, washed again, and transferred to a solution containing 1% avidin-conjugated horseradish peroxidase complex (Vectastain ABC-Elite Standard kit, Vector Laboratories PK-6100) and 1%Triton X-100 for 12 h. After rinsing in 0.1 M PB, slices were incubated with a solution containing 0.036%DAB (Sigma-Aldrich, D5637-5G), 0.006% NiCl2 (Sigma-Aldrich, 223387–25G) and 0.008% CoCl2 (Sigma-Aldrich, C8661-25G), and developed by addition of 0.01% hydrogen peroxide. .. DAB developed slices weremounted inMowiol and cured for at least 24 h, before visualization using an Olympus BX61 widefield microscope.

Incubation:

Article Title: The Novel Omega-6 Fatty Acid Docosapentaenoic Acid Positively Modulates Brain Innate Immune Response for Resolving Neuroinflammation at Early and Late Stages of Humanized APOE-Based Alzheimer's Disease Models
Article Snippet: Vector biotinylated goat anti-rabbit (BA-1000) antibody (1:1,200) was used with 1.5% normal goat serum and 3% bovine serum albumin (BSA) in TBS. .. Then sections were incubated in secondary antibody followed by VECTASTAIN ABC Elite Standard Kit (PK-6100, Vector Labs) at 37°C for one hour. .. Sections were developed using a Peroxidase/Diaminobezidiene Metal Enhance Substrate kit (Cat. 34065; Pierce, Rockford, IL.).

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